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Electrochemical Detection of Heparin Based on Its Interaction with Light Green
Author(s) -
Sun Wei,
Ding Yaqin,
Wang Qingxiang,
Jiao Kui
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200603511
Subject(s) - linear sweep voltammetry , chemistry , heparin , buffer solution , detection limit , electrochemistry , analytical chemistry (journal) , selectivity , cyclic voltammetry , prime (order theory) , voltammetry , chromatography , redox , linear relationship , electrode , inorganic chemistry , mathematics , catalysis , organic chemistry , biochemistry , combinatorics , statistics
The determination of heparin with linear sweep voltammetry has been developed in this paper based on its interaction with light green (LG). In pH 2.0 Britton–Robinson (B–R) buffer solution, LG has a sensitive linear sweep voltammetric reduction peak at −0.41 V (vs. SCE) and heparin can react with LG to form an ion‐association electro‐active complex, which resulted in the decrease of the peak current of the reaction solution. Under the selected experimental conditions the decrease of peak current was directly proportional to the concentration of heparin in the range of 0.8–20.0 μg/mL with the linear regression equation as Δ i $\rm{ {_{p}^{\prime\prime }}}$ (nA)=79.12 C −101.44 ( n =14, γ =0.996) and the detection limit as 0.28 μg/mL (3 σ ). The effects of coexisting substances on the determination of heparin were investigated and the results showed that this method had good selectivity. The method was further applied to the determination of heparin content in heparin samples with satisfactory results and good recovery.