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Amperometric Glucose Biosensor Based on Rhodium Dioxide‐Modified Carbon Ink
Author(s) -
Kotzian Petr,
Brázdilová Petra,
Řezková Soňa,
Kalcher Kurt,
Vytřas Karel
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200503549
Subject(s) - amperometry , biosensor , detection limit , glucose oxidase , chemistry , electrode , nafion , chromatography , flow injection analysis , carbon paste electrode , linear range , analytical chemistry (journal) , electrochemistry , cyclic voltammetry , biochemistry
Abstract An amperometric method for the determination of glucose using a screen printed carbon electrode is reported. The electrode material was bulk modified with rhodium dioxide and the enzyme glucose oxidase immobilized in a Nafion‐film on the electrode surface and investigated for its ability to serve as a detector of glucose in flow injection analysis. The sensor exhibited a linear increase of the amperometric signal with the concentration of glucose in the range of 1–250 mg L −1 with a detection limit (evaluated as 3 σ ) of 0.2 mg L −1 under optimized flow rate of 0.4 mL min −1 in 0.1 M phosphate buffer (pH 7.5) carrier. At the potential applied (−0.2 V vs. Ag/AgCl), interferences from redox species present in the sample matrix were negligible. The biosensor reported here retained its activity for more than 40 injections or 4 months of storage at 6 °C. The RSD was determined as 1.8% for a glucose concentration of 25 mg L −1 ( n =5) with a typical response time of about 28 s.