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Nitric Oxide Biosensors Based on the Immobilization of Hemoglobin on Mesoporous Titania Electrodes
Author(s) -
Topoglidis Emmanuel,
Campbell Colin J.,
Cass Anthony E. G.,
Durrant James R.
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200503473
Subject(s) - nitric oxide , mesoporous material , electrode , nanocrystalline material , biosensor , materials science , adsorption , hemoglobin , protein adsorption , oxide , electrochemistry , conductivity , chemical engineering , nanotechnology , inorganic chemistry , chemistry , catalysis , organic chemistry , metallurgy , engineering
A nanocrystalline TiO 2 film is an electrode material with large surface area which allows high levels of protein adsorption without loss of protein structure or activity. As an optically transparent semiconductor, titania can be used to carry out direct spectroelectrochemistry of proteins such as hemoglobin. We demonstrate that the high protein loading and the optical transparency and electrical conductivity of the Hb/TiO 2 films allow the optical and/or electrochemical sensing of nitric oxide. In particular we demonstrate the nitric oxide cycle of oxyhemoglobin immobilized on TiO 2 films and use it to electrochemically measure micromolar levels of nitric oxide.