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Direct Electrochemistry of Multi‐Copper Oxidases at Carbon Nanotubes Noncovalently Functionalized with Cellulose Derivatives
Author(s) -
Zheng Wei,
Li Qingfen,
Su Lei,
Yan Yiming,
Zhang Jun,
Mao Lanqun
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200503444
Subject(s) - electrochemistry , copper , cellulose , carbon nanotube , chemistry , carbon fibers , combinatorial chemistry , materials science , nanotechnology , organic chemistry , electrode , composite number , composite material
Abstract This study describes the direct electron transfer of multi‐copper oxidases, i.e., laccase (from Trametes versicolor ) and bilirubin oxidase (BOD, from Myrothecium verrucaria ) at multiwalled carbon nanotubes (MWNTs) noncovalently functionalized with biopolymers of cellulose derivatives, i.e., hydroxyethyl cellulose (HEC), methyl cellulose (MC), and carboxymethyl cellulose (CMC). The functionalization of the MWNTs with the cellulose derivatives is found to substantially solubilize the MWNTs into aqueous media and to avoid their aggregation on electrode surface. Under anaerobic conditions, the redox properties of laccase and BOD are difficult to be defined with cyclic voltammetry at either laccase/MWNT‐modified or BOD/MWNT‐modified electrodes. The direct electron transfer properties of laccase and BOD are thus studied in terms of the bioelectrocatalytic activities of the laccase/MWNT‐modified and BOD/MWNT‐modified electrodes toward the reduction of oxygen and found to be facilitated at the functionalized MWNTs. The possible application of the laccase‐catalyzed O 2 reduction at the laccase/MWNT‐modified electrode is illustrated by constructing a CNT‐based ascorbate/O 2 biofuel cell with the MWNT‐modified electrode as the anode for the oxidation of ascorbate biofuel.