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Peroxynitrite/Nitric Oxide Balance in Ischemia/Reperfusion Injury‐Nanomedical Approach
Author(s) -
Kubant Ruslan,
Malinski Christopher,
Burewicz Andrzej,
Malinski Tadeusz
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200503436
Subject(s) - peroxynitrite , nitric oxide , oxidative stress , ischemia , in vivo , reperfusion injury , endothelium , chemistry , pharmacology , medicine , biochemistry , biology , microbiology and biotechnology , enzyme , superoxide
Electrochemical nanosensors were used to simultaneously monitor in vitro (a single endothelial cell) and in vivo (vasculature of rat) the concentrations of NO (vasorelaxant), cytotoxic O $\rm{ {_{2}^{-}}}$ (oxidative stress) and ONOO − (nitroxidative stress). A balance of [NO]/[ONOO − ]=( K ) was applied as the diagnostic marker of dysfunctional endothelium and cardiovascular disease. In the isolated endothelium of normotensive rats, K= 2.8±0.1 while in hypertensive rats, K =0.4±0.1. During ischemia, K dropped from 7±1 to 1.4±0.2 and further decreased to 0.05±0.01 during reperfusion. The edema and vasoconstriction, indicators of vascular injury, correlated directly with the decrease in K.