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NADH Electrooxidation Using Bis(1,10‐phenanthroline‐5,6‐dione)(2,2′‐bipyridine)ruthenium(II)‐Exchanged Zirconium Phosphate Modified Carbon Paste Electrodes
Author(s) -
Santiago Mitk'El B.,
Vélez Meredith M.,
Borrero Solmarie,
Díaz Agustín,
Casillas Craig A.,
Hofmann Cristina,
Guadalupe Ana R.,
Colón Jorge L.
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200503432
Subject(s) - ruthenium , intercalation (chemistry) , cyclic voltammetry , zirconium phosphate , phenanthroline , inorganic chemistry , electrocatalyst , chemistry , carbon paste electrode , nuclear chemistry , 2,2' bipyridine , phosphate , electrochemistry , catalysis , electrode , crystallography , organic chemistry , crystal structure
We present a carbon paste electrode (CPE) modified using the electron mediator bis(1,10‐phenanthroline‐5,6‐dione)(2,2′‐bipyridine)ruthenium(II) ([Ru(phend) 2 bpy] 2+ ) exchanged into the inorganic layered material zirconium phosphate (ZrP). X‐Ray powder diffraction showed that the interlayer distance of ZrP increases upon [Ru(phend) 2 bpy] 2+ intercalation from 10.3 Å to 14.2 Å. The UV‐vis and IR spectroscopies results showed the characteristic peaks expected for [Ru(phend) 2 bpy] 2+ . The UV‐vis spectrophotometric results indicate that the [Ru(phend) 2 bpy] 2+ concentration inside the ZrP layers increased as a function of the loading level. The exchanged [Ru(phend) 2 bpy] 2+ exhibited luminescence even at low concentration. Modified CPEs were constructed and analyzed using cyclic voltammetry. The intercalated mediator remained electroactive within the layers ( E °′=–38.5 mV vs. Ag/AgCl, 3.5 M NaCl) and electrocatalysis of NADH oxidation was observed. The kinetics of the modified CPE shows a Michaelis–Menten behavior. This CPE was used for the oxidation of NADH in the presence of Bakers' yeast alcohol dehydrogenase. A calibration plot for ethanol is presented.