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Separation and Detection of Nitrophenols at Capillary Electrophoresis Microchips with Amperometric Detection
Author(s) -
Fischer Jan,
Barek Jirí,
Wang Joseph
Publication year - 2006
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200503393
Subject(s) - capillary electrophoresis , amperometry , chromatography , chemistry , analytical chemistry (journal) , electrode , electrochemistry
Abstract A miniaturized analytical system for the separation and amperometric detection of toxic nitrophenols, based on the coupling of a micromachined capillary electrophoresis (CE) chip with a glassy carbon detector is described. This microsystem enables a rapid (120 s/sample) simultaneous determination of five priority nitrophenolic pollutants (2‐nitrophenol, 3‐nitrophenol, 4‐nitrophenol, 2,4‐dinitrophenol, and 2‐methyl‐4,6‐dinitrophenol). These compounds can be detected down to the 1×10 −5 M level using a 15 mM phosphate buffer pH 7.2 (containing 1.3 mM α‐cyclodextrin) as running solution on 77 mm long microchannel by applying a separation voltage of 3000 V and a negative potential of −0.7 V (vs. Ag /AgCl wire). Applicability to ground water samples was demonstrated.