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Electrochemical Study of the Lipid‐Transfer Protein
Author(s) -
Gorjanović Stanislava,
Beljanski Miloš V.,
Sužnjević Desanka
Publication year - 2005
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200503315
Subject(s) - plant lipid transfer proteins , chemistry , polarography , electrochemistry , analyte , metal ions in aqueous solution , cyclic voltammetry , differential pulse voltammetry , anodic stripping voltammetry , ion , chromatography , inorganic chemistry , biochemistry , electrode , organic chemistry , gene
Abstract Electrochemical study of barley grain lipid‐transfer protein (LTP) revealed that it may belong to the metal‐binding protein class. Using differential pulse polarography the presence of Cu(II) and Zn(II) ions in the native LTP structure was proved, as well as its affinity for binding Ni(II) ion. Application of a more sensitive electroanalytical technique, such as anodic stripping voltammetry with analyte preconcentration, revealed the presence of Pb(II) and Cd(II) ions and also enabled the following Hg(II) ion binding. Possible biological role of LTP in plant stress response and its contribution to barley phytoextraction potential are discussed.