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Electrochemical Study of the Interactions of DNA with Redox‐Active Molecules Based on the Immobilization of dsDNA on the Sol‐Gel Derived Nano Porous Hydroxyapatite‐Polyvinyl Alcohol Hybrid Material Coating
Author(s) -
Wang HuaiSheng,
Wang GuiXiang,
Pan QianXiu
Publication year - 2005
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200503314
Subject(s) - polyvinyl alcohol , cyclic voltammetry , redox , electrochemistry , molecule , coating , scanning electron microscope , adsorption , electrode , nuclear chemistry , chemistry , materials science , chemical engineering , inorganic chemistry , nanotechnology , organic chemistry , composite material , engineering
A novel type of sol‐gel inorganic‐organic hybrid material coated on glassy carbon electrode used for immobilization of double‐stranded DNA (dsDNA) and study of dsDNA with redox‐active molecules was developed. The hybrid material coating was produced by sol‐gel method with nano hydroxyapatite (HAp)‐polyvinyl alcohol (PVA). The optimum composition of the hybrid material was first examined, and the morphology of the nano HAp‐PVA coatings was investigated with the help of Scanning Electron Microscope (SEM). DsDNA was immobilized in/on the nano HAp‐PVA hybrid coatings by adsorption and the characteristics of the dsDNA/HAp‐PVA/GCE were studied by cyclic voltammetry (CV) using the probes of Co(phen) $\rm{ {_{3}^{3+}}}$ and Fe(CN) $\rm{ {_{6}^{3-}}}$ . The results indicate that the dsDNA can be immobilized on the nano porous HAp‐PVA coating effectively and its stability can satisfy the necessity of study on the interactions of dsDNA with redox‐active molecules on the electrode surface. Co(bpy) $\rm{ {_{3}^{3+}}}$ and Co(phen) $\rm{ {_{3}^{3+}}}$ were used as the model molecule to study the interactions of dsDNA with redox‐active molecules. Information such as ratio ( K Ox / K Red ) of the binding constant for the oxidized and reduced forms of a bound species, interaction mode, including change in the mode of interaction, and “limiting” ratio K $\rm{ {_{Ox}^{^{o}}}}$ / K $\rm{ {_{Red}^{^{o}}}}$ at zero ionic strength ( μ ) can be obtained using dsDNA/HAp‐PVA/GCE with about 2 μg of DNA samples.