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Barbituric Acid Sensor Based on Molecularly Imprinted Polymer‐ Modified Hanging Mercury Drop Electrode
Author(s) -
Prasad Bhim Bali,
Lakshmi Dhana
Publication year - 2005
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200403226
Subject(s) - barbituric acid , hanging mercury drop electrode , chemistry , molecularly imprinted polymer , detection limit , electrode , supporting electrolyte , dropping mercury electrode , inorganic chemistry , electrolyte , electrochemistry , chromatography , voltammetry , selectivity , organic chemistry , catalysis
A barbituric acid sensor based on molecularly imprinted polymer‐modified hanging mercury drop electrode is reported for sensitive and selective analysis in aqueous and blood plasma samples. It was developed by coating directly the barbituric acid‐ imprinted polymer, which is prepared from melamine and chloranil, onto the surface of a hanging mercury drop electrode under charge‐transfer interaction at +0.3 V (vs. Ag/AgCl) in Model 303A electrode system connected with a polarographic analyzer/stripping voltammeter (PAR Model 264A). The binding event of barbituric acid was detected in the imprinted polymer layer through differential pulse, cathodic stripping voltammetric (DPCSV) signal at optimized operational conditions [accumulation potential +0.3 V (vs. Ag/AgCl), accumulation time 120 s, pH 7.0 (without supporting electrolyte), scan rate 10 mV s −1 , pulse amplitude 25 mV]. The limit of detection for barbituric acid was found to be 0.54 ppm (RSD=3.7%, S/N =3). The detection was feasible without any problem of matrix effect or cross reactivity in the linearity range of concentration varying from 0.50 to 125.00 ppm of barbituric acid.