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Fabricating of Acetylcholine Biosensor by a Layer‐by‐layer Deposition Technique for Determining Trichlorfon
Author(s) -
Shi Haibin,
Zhao Zixia,
Song Zhao,
Huang Jiadong,
Yang Yu,
Anzai Junichi,
Osa Tetsuo,
Chen Qiang
Publication year - 2005
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200403223
Subject(s) - biosensor , layer by layer , layer (electronics) , chemistry , detection limit , deposition (geology) , chromatography , chloride , organophosphate , nanotechnology , materials science , pesticide , organic chemistry , paleontology , sediment , agronomy , biology
An acetylcholine (ACh) biosensor has been fabricated with bienzymes/poly(diallyldimethylammonium chloride) (PDDA) multilayer film‐modified platinum (Pt) electrodes by a layer‐by‐layer technique (LBL). The ACh biosensor was optimized and the properties are described. This ACh biosensor was used for the detection of organophosphate pesticide trichlorfon. The detection limits (found 0.001 μg/mL for trichlorfon) make it possible to detect the pollutants. This simple protocol of biosensor preparation, high sensitivity and stability are very promising for the determination of environmental pollutants in field conditions.