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Determination of Active Components in St. John's Wort (Hypericum Perforatum) by Capillary Electrophoresis with Electrochemical Detection
Author(s) -
Peng Youyuan,
Yuan Jianjun,
Ye Jiang
Publication year - 2005
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200403219
Subject(s) - hypericum perforatum , capillary electrophoresis , chromatography , hyperforin , analyte , hypericum , working electrode , chemistry , extraction (chemistry) , hypericin , electrophoresis , electrochemistry , electrode , traditional medicine , medicine , pharmacology
St. John's Wort ( Hypericum perforatum ) is a medicinal plant which has been known in traditional medicine as an anti‐inflammatory and healing agent. Nowadays, the alcoholic extract of its aerial parts finds wide application for its antidepressant activity. A capillary electrophoresis method for the identification of its constituents using electrochemical detection was developed. Effects of several important factors were investigated to acquire the optimum conditions. The working electrode was a 300 μm carbon disc electrode at a working potential of +0.90 V (vs. SCE). The analytes can be well separated within 20 min in a 75 cm length fused‐sillica capillary at a separation of 16 kV in 50 mmol L −1 borate buffer (pH=9.0). The current response was linear over about three orders of magnitude with detection limits ( S/N =3) ranging from 0.2 to 0.5 mg L −1 for all the analytes. The method was successfully used in the analysis of Hypericum perforatum with relatively simple extraction procedures, and the assay results were satisfactory.