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An Amperometric Enzyme Biosensor Fabricated from Polyaniline Nanoparticles
Author(s) -
Morrin Aoife,
Ngamna Orawan,
Killard Anthony J.,
Moulton Simon E.,
Smyth Malcolm R.,
Wallace Gordon G.
Publication year - 2005
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200403185
Subject(s) - biosensor , polyaniline , materials science , nanoparticle , adsorption , nanotechnology , scanning electron microscope , conductive polymer , horseradish peroxidase , chemical engineering , glassy carbon , amperometry , polymer , electrode , electrochemistry , cyclic voltammetry , chemistry , organic chemistry , composite material , engineering , polymerization , enzyme
The biosensor described here uses a novel aqueous‐based nanoparticulate polyaniline (PANI), doped with dodecylbenzenesulfonic acid (DBSA). The nanoparticles were applied to a glassy carbon electrode surface by electrodeposition techniques, and horseradish peroxidase (HRP) was subsequently electrostatically adsorbed to the nanoparticle‐modified surface. This biosensor format was demonstrated for H 2 O 2 sensing. Electrodeposited polymer nanoparticles resulted in highly ordered conductive nanostructured films, which were examined by scanning electron microscopy (SEM), atomic force microscopy (AFM), profilometry and spectroelectrochemistry. The surface of the films were characterized by a uniform array of nanoparticulate PANI (nanoPANI/DBSA) nodules and were shown to have a thickness of 350 nm. Physical techniques have shown that the nanofilms possess properties which allow for uniform electrostatic adsorption of protein to take place. This effective biosensor format, exhibits higher signal‐to‐background ratios and shorter response times than previous PANI biosensor configurations.