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Highly‐Sensitive Determination of 6‐Mercaptopurine and Its Metabolites by Electrochemical Reductive Desorption Measurements
Author(s) -
Sato Yukari,
Ishikawa Yayoi,
Matsuura Hiroaki,
Uosaki Kohei,
Mizutani Fumio,
Niwa Osamu
Publication year - 2005
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200403165
Subject(s) - detection limit , chemistry , desorption , electrochemistry , metabolite , chromatography , chemisorption , electrode , adsorption , organic chemistry , biochemistry
The electrochemical detection of 6‐mercaputopurine (6‐MP), which is one of the most well‐known drugs of antimetabolites, and its analogues was studied. The presented method is based on chemisorption (accumulation)/desorption processes of drugs onto/from the Au electrode surface. Using this method, we succeeded in detecting 6‐MP even in very diluted solution (concentration of 6‐MP: 1×10 −8 M). The lower detection limit of 6‐MP was considerably improved compared with the previous HPLC studies by using our proposed system. Desorptive potentials of 6‐MP‐metabolites and its analogues were different from those of 6‐MP. This provides the possibility of simultaneous detection of 6‐MP and its typical metabolites.