Premium
An Electrochemical Biosensor with Cholesterol Oxidase/ Sol‐Gel Film on a Nanoplatinum/Carbon Nanotube Electrode
Author(s) -
Qiaocui Shi,
Tuzhi Peng,
Yunu Zhu,
Yang Catherine F.
Publication year - 2005
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200403162
Subject(s) - cholesterol oxidase , biosensor , electrode , carbon nanotube , detection limit , platinum , electrochemistry , graphite , hydrogen peroxide , materials science , nuclear chemistry , nafion , linear range , inorganic chemistry , catalysis , chemistry , nanotechnology , chromatography , organic chemistry , composite material , enzyme
The carbon nanotubes decorated nanoplatinum (CNT‐Pt) were prepared using a chemical reduction method and a novel base electrode was constructed by intercalating CNT‐Pt on the surface of a waxed graphite electrode. The results showed that the nano‐particles of platinum at a waxed graphite electrode exhibits high catalytic activity for the reduction of hydrogen peroxide. The cholesterol oxidase (ChOx), chosen as a model enzyme, was immobilized with sol‐gel on the CNT‐Pt base electrode to construct a biosensor. The current response of the biosensor for cholesterol was very rapid (<20 s). The linear range for cholesterol measurement was 4.0×10 −6 mol/L −1.0×10 −4 mol/L with a detection limit of 1.4×10 −6 mol/L. The experiments also showed that the ChOx/sol‐gel/CNT‐Pt biosensor was sensitive and stable in detecting cholesterol in serum samples.