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An Inexpensive Biosensor for Uric Acid Determination in Human Serum by Flow‐Injection Analysis
Author(s) -
Dutra R. F.,
Moreira K. A.,
Oliveira M. I. P.,
Araújo A. N.,
Montenegro M. C. B. S.,
Filho J. L. L.,
Silva V. L.
Publication year - 2005
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200403142
Subject(s) - biosensor , flow injection analysis , tetracyanoquinodimethane , uric acid , chromatography , chemistry , amperometry , relative standard deviation , cyclic voltammetry , detection limit , electrode , biochemistry , organic chemistry , electrochemistry , molecule
An inexpensive and easy to construct miniaturized biosensor is described for the determination of uric acid in biological fluids. The amperometric biosensor was prepared by using a carbon paste electrode prepared with uricase from Arthrobacter globiforms and tetracyanoquinodimethane as electron transfer mediator. When incorporated into a flow‐injection system it was enabled to perform 50 measurements/h of uric acid in the analytical range of 1–100 μmol dm −3 with a relative standard deviation of 0.20% ( n =14). The system was applied to human serum samples analysis providing good data correlation with those obtained by the reference spectrophotometric method. A linear relationship AM (μmol dm −3 )=1.02 (±0.05) SP (μmol dm −3 ) −0.12 (±0.13) was obtained evidencing the absence of significant error. The constructed biosensor was successfully used for at least four months (250 assays) with only a 13% of decrease in the enzymatic activity.