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Modelling of the Potentiometric Response of ENFETs Based on Enzymatic Multilayer Membranes
Author(s) -
Aouni F.,
Mlika R.,
Martelet C.,
Ouada H. Ben,
JaffrezicRenault N.,
Soldatkin A. P.
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200403079
Subject(s) - membrane , biosensor , potentiometric titration , silanol , chemistry , isfet , buffer (optical fiber) , urea , nafion , amine gas treating , diffusion , urease , chromatography , chemical engineering , analytical chemistry (journal) , electrode , field effect transistor , biochemistry , electrochemistry , organic chemistry , transistor , thermodynamics , telecommunications , physics , voltage , quantum mechanics , computer science , engineering , catalysis
Based on the modified site‐binding model, the response of urea‐sensitive enzymatic field effect transistors (ENFETs) is fitted. The effect of two types of surface sites (silanol and amine sites) and of additional polymeric permselective membrane on the sensitivity of the biosensor is discussed. It is found that the dependence of the slope of the sensor response versus pUrea, on the buffer concentration and on the diffusion of the urea in the enzymatic membrane can be translated by a parameter α that depends on the membrane composition. Moreover, the enhancement of the sensor sensitivity by deposition of additional permselective membranes can be illustrated by a parameter δ that depends on the association of additional permselective membranes and a buffer; the higher values of parameter δ are obtained with a Nafion membrane associated with a phosphate buffer.