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Potentiometric Biosensors Based on ISFETs and Immobilized Cholinesterases
Author(s) -
Dzyadevych Sergei V.,
Arkhypova Valentyna N.,
Martelet Claude,
JaffrezicRenault Nicole,
Chovelon JeanMarc,
El'skaya Anna V.,
Soldatkin Alexey P.
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200403075
Subject(s) - biosensor , chemistry , chromatography , hypochlorite , carbamate , reproducibility , potentiometric titration , immobilized enzyme , enzyme , organic chemistry , biochemistry , ion
This paper describes the authors' achievements in the development, investigation, and application of cholinesterase biosensors based on ISFETs. Various biosensors for determination of concentrations of different toxic substances (organophosphorous and carbamate pesticides, hypochlorite, glycoalkaloids) were designed on the basis of reversible and/or irreversible enzyme inhibition effects. The main analytical characteristics of the biosensors developed have been studied under different conditions and optimal experimental protocols for toxic substances determination have been proposed. Most of these biosensors show a high reproducibility and a good operational and storage stability. A quite good correlation with results obtained through routinely used standard methods as HPLC has been shown.