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Gold Nanoparticle‐Catalyzed Silver Electrodeposition on an Indium Tin Oxide Electrode and Its Application in DNA Hybridization Transduction
Author(s) -
Lee Thomas MingHung,
Cai Hong,
Hsing IMing
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200403039
Subject(s) - indium tin oxide , electrode , colloidal gold , nanoparticle , materials science , electrochemistry , catalysis , inorganic chemistry , hydroquinone , nanotechnology , chemical engineering , silver nanoparticle , chemistry , thin film , organic chemistry , engineering
Abstract In this work, we report a simple, rapid and sensitive approach for the electrochemical gold nanoparticle‐based DNA detection with an electrocatalytic silver deposition process. The catalytic and preferential silver electrodeposition on gold nanoparticle surfaces using an indium tin oxide (ITO) electrode at certain potentials, without any chemical pretreatments of the electrode, is demonstrated. More importantly, the application of this methodology for hybridization transduction is explored. The ITO electrode surface is first coated with an electroconductive polymer, poly(2‐aminobenzoic acid), to enable the chemical attachment of avidin molecules for the subsequent probe immobilization. The hybridization of the target with the probe in turn permits the binding of the gold nanoparticle labels to the transducer surface via biotin‐streptavidin interaction. The amount of bound gold labels, which is proportional to the amount of the target, is determined by the electrocatalytic silver deposition process. A significant improvement of the signal‐to‐background ratio is achieved with this scheme compared to the conventional chemical hydroquinone‐based silver deposition process.