Detection Mechanism of Metallized Carbon Epoxy Oxidase Enzyme Based Sensors | Zendy

Khurana Manpreet K. | Zendy; Winlove C. Peter | Zendy; O'Hare Danny | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Premium

Detection Mechanism of Metallized Carbon Epoxy Oxidase Enzyme Based Sensors

Author(s) -

Khurana Manpreet K.,

Winlove C. Peter,

O'Hare Danny

Publication year - 2003

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.200390123

Subject(s) - biosensor , biocompatibility , analyte , epoxy , biocompatible material , chemistry , coating , selectivity , membrane , enzyme , nanotechnology , materials science , biochemistry , chromatography , organic chemistry , biomedical engineering , catalysis , medicine

The coenzyme FAD has been identified to play an important role in the detection mechanism of oxidase enzyme based biosensors. Incorporating FAD into the carbon composite improved sensitivity to H 2 O 2 consequently increasing sensitivity to the respective analyte. The amount of active enzyme also increased thus enhancing the overall performance of the sensors. Polycarboxybetaine (PCB) has been used as a biocompatible membrane coating. The PCB coated sensors gave reproducible calibrations in protein solutions, which has been shown to be a valid protocol for testing biocompatibility. The importance of reporting selectivity in a manner which indicates the “fitness of purpose” of biosensors has been discussed.

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here

Accelerating Research