Premium
Detection Mechanism of Metallized Carbon Epoxy Oxidase Enzyme Based Sensors
Author(s) -
Khurana Manpreet K.,
Winlove C. Peter,
O'Hare Danny
Publication year - 2003
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200390123
Subject(s) - biosensor , biocompatibility , analyte , epoxy , biocompatible material , chemistry , coating , selectivity , membrane , enzyme , nanotechnology , materials science , biochemistry , chromatography , organic chemistry , biomedical engineering , catalysis , medicine
The coenzyme FAD has been identified to play an important role in the detection mechanism of oxidase enzyme based biosensors. Incorporating FAD into the carbon composite improved sensitivity to H 2 O 2 consequently increasing sensitivity to the respective analyte. The amount of active enzyme also increased thus enhancing the overall performance of the sensors. Polycarboxybetaine (PCB) has been used as a biocompatible membrane coating. The PCB coated sensors gave reproducible calibrations in protein solutions, which has been shown to be a valid protocol for testing biocompatibility. The importance of reporting selectivity in a manner which indicates the “fitness of purpose” of biosensors has been discussed.