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Microchip Separation and Electrochemical Detection of Amino Acids and Peptides Following Precolumn Derivatization with Naphthalene‐2,3‐dicarboxyaldehyde
Author(s) -
Wang Joseph,
Chen Gang,
Pumera Martin
Publication year - 2003
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200390106
Subject(s) - analyte , chemistry , chromatography , detection limit , reagent , microfluidics , amino acid , calibration curve , electrode , nanotechnology , materials science , organic chemistry , biochemistry
An integrated microfluidic device is used to derivatize, separate, and amperometrically detect amino acids and peptides in the presence of naphthalene‐2,3‐dicarboxyaldehyde (NDA). The integrated system offers a rapid (4 min) simultaneous measurements of 5 amino acids (Arg, Lys, Gly, Cys, PhenA) down to the 3.2 μM level in connection to a precolumn reaction chamber, an electrophoretic separation channel, and an end‐column thick‐film carbon‐electrode detector. The effect of the separation voltage, detection potential, reagent concentration, and other variables on the response is examined. Calibration and precision experiments indicate a linear and reproducible response. Applicability for the separation and detection of small peptides is demonstrated. Such on‐chip generation of electroactive products offers great promise for detecting other nonelectroactive analytes.