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Development of a Multiresidue Liquid Chromatography Method with Electrochemical Detection (HPLC‐ECD) for the Determination of Macrolide Antibiotics
Author(s) -
González de la Huebra María José,
Bordin Guy,
Rodríguez Adela Rosa
Publication year - 2003
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200390056
Subject(s) - chromatography , oleandomycin , chemistry , roxithromycin , amperometry , high performance liquid chromatography , elution , josamycin , calibration curve , detection limit , electrochemistry , antibiotics , electrode , biochemistry , erythromycin
A multiresidue high performance liquid chromatography procedure with electrochemical detection (amperometric) (HPLC‐ECD) using a C18 narrow bore column was fully optimized for the separation of a standard mixture of macrolides. Up to five macrolides, josamycin (JOS), kitasamycin (KIT), rosamicin (ROS) roxithromycin (ROX) and oleandomycin (OLE), the latter being selected as internal standard for the quantification, were properly separated using an isocratic elution mode. The influence of classical chromatographic and instrumental parameters was investigated in order to optimize them before the calibration assay. The method was succesfully applied to the determination of ROS and ROX in human urine.