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Organic Phase PPO Biosensors Prepared by Multilayer Deposition of Enzyme and Alginate Through Avidin‐Biotin Interactions
Author(s) -
Cosnier S.,
Mousty C.,
de Melo J.,
Lepellec A.,
Novoa A.,
Polyak B.,
Marks R. S.
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200303084
Subject(s) - biosensor , chlorobenzene , catechol , chemistry , polyphenol oxidase , glucose oxidase , solvent , substrate (aquarium) , dichloromethane , avidin , immobilized enzyme , microelectrode , acetonitrile , combinatorial chemistry , biotin , organic chemistry , electrode , enzyme , peroxidase , biochemistry , catalysis , oceanography , geology
Films of electrogenerated polypyrrole and hydrophilic alginate, both functionalized with biotin moieties, were used to allow for the transfer of polyphenol oxidase activity in organic media. Enzyme electrodes, based on multilayered structures, were protected at the molecular level by the affinity binding of alginate as a hydrophilic additive, and were then transferred into chlorobenzene, dichloromethane, chloroform, ethyl acetate or acetonitrile. The biosensor performance for the detection of catechol at −0.2 V was investigated, highlighting the main influence of the hydrophobicity of the solvent and, to a lesser extent, the dielectric constant. The effect of the substrate hydrophobicity on the biosensor response was examined in chlorobenzene.