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Development of a Rapid Single‐Drop Analysis Biosensor for Screening of Phenanthrene in Water Samples
Author(s) -
Moore Eric J.,
Kreuzer Mark P.,
Pravda Miloslav,
Guilbault George G.
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200303033
Subject(s) - detection limit , linear sweep voltammetry , phenanthrene , linear range , biosensor , tap water , chromatography , analytical chemistry (journal) , chemistry , reproducibility , electrode , materials science , cyclic voltammetry , environmental chemistry , environmental science , nanotechnology , environmental engineering , electrochemistry
Detection techniques for biosensors often require bulky instruments or cells that are not feasible for in‐field analysis. Our single‐drop cell design, optimized in this work, comprised a screen‐printed three‐electrode (SPE), strip in horizontal position onto which a volume of 100 μL of sample or substrate solution was placed to ensure electrical contact (complete circuit). Together with optimized linear sweep voltammetry (LSV), parameters for the detection of the enzyme alkaline phosphatase (AP), the system was applied to a biosensor for the analysis of polycyclic aromatic hydrocarbons (PAHs), in environmental samples. A limit of detection (LOD), of 0.15 ppb was achieved for a model system with an IC 50 value of 0.885 ppb and a linear range (LR), of 0.2–10 ppb. Application of the single drop analysis (SDA), format to a PAH biosensor gave a LOD of 1.4 ppb for detection of phenanthrene with an IC 50 value of 29.3 ppb and linear range of 2–100 ppb. Proof of concept is shown with spiked sample analysis of phenanthrene in matrices such as sea, river and tap water.