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Glucose Microbiosensor Based on MnO 2 and Glucose Oxidase Modified Carbon Fiber Microelectrode
Author(s) -
Hocevar Samo B.,
Ogorevc Bozidar,
Schachl Klemens,
Kalcher Kurt
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200303019
Subject(s) - glucose oxidase , amperometry , microelectrode , nafion , electrochemistry , detection limit , biosensor , chemistry , materials science , analytical chemistry (journal) , chemical engineering , chromatography , electrode , nanotechnology , engineering
A glucose microbiosensor has been developed using electrochemical codeposition of glucose oxidase (GOx) along with MnO 2 as mediator, onto a single carbon fiber microelectrode. A two‐step deposition of only MnO 2 initially and then of MnO 2 in the presence of GOx has been introduced to ensure appropriate activity of the mediator. Several parameters such as deposition potential and time, concentration levels etc. have been characterized and optimized. A thin Nafion film was applied as an immobilization/encapsulation and interference‐free protective layer. The proposed microbiosensor was employed as an amperometric glucose detector at pH 7.5 at an operating potential of +0.58 V (vs. Ag/AgCl). The microbiosensor is characterized by a well‐reproducible amperometric response, linear signal‐to‐glucose concentration range from 1.5 mmol L −1 to 15 mmol L −1 , and a limit of detection ( S/N =3) of 0.8 mmol L −1 . The microbiosensor exhibits good stability over more than ten hours of continuous measurement.