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Biosensor for Hepatitis B Virus DNA PCR Product and Electrochemical Study of the Interaction of Di(2,2′‐bipyridine)osmium(III) with DNA
Author(s) -
Zhao Hongtao,
Ju Huangxian
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200303013
Subject(s) - dna , osmium , electrochemistry , intercalation (chemistry) , chemistry , dna–dna hybridization , hepatitis b virus , biosensor , glassy carbon , supporting electrolyte , covalent bond , selectivity , binding constant , electrode , inorganic chemistry , binding site , biochemistry , cyclic voltammetry , virus , ruthenium , organic chemistry , biology , virology , catalysis
The strategy for electrochemical detection of HBV DNA PCR product (181 bps) was designed by covalently immobilizing single‐stranded HBV DNA on preoxidized glassy carbon electrode surface. The immobilization of single stranded DNA was verified by AC impedance spectra. The following hybridization reaction on surface was evidenced by electrochemical methods using [Os(bpy) 2 Cl 2 ] + as an electroactive indicator. The interactions of [Os(bpy) 2 Cl 2 ] + with calf thymus single and double stranded DNA immobilized on preoxidized glassy carbon electrodes were studied. [Os(bpy) 2 Cl 2 ] + could bind preferentially to the duplex DNA by intercalating to base pairs. The intrinsic binding constant of [Os(bpy) 2 Cl 2 ] + with calf thymus DNA was calculated to be 1.21×10 4 M −1 . Using [Os(bpy) 2 Cl 2 ] + as an electrochemical hybridization indicator, the HBV DNA sensor has been used to detect qualitatively target HBV DNA in solution with high sensitivity and selectivity.