Electrochemical and Spectroelectrochemical Studies on Pyridoxine Hydrochloride Using a Poly(methylene blue) Modified Electrode

The electrochemical redox processes of pyridoxine hydrochloride (VB 6 ) at a poly(methylene blue) film modified glass carbon electrode (PMBE) in a phosphate buffer solution (PBS, pH 8.0) were studied by cyclic voltammetry. The VB 6 electrode reaction with quasi‐reversible characteristics was diffusion‐controlled at low scan rates and adsorption‐controlled at high scan rates. The anodic peak current positive to 0.6 V (vs. SCE) was found to be proportional to the concentration of VB 6 in the range of 0.010 to 1.03 mg⋅mL −1 with a detection limit of 1.34 μg mL −1 . Fluorescence and UV‐vis absorption spectroelectrochemical measurements suggest that the pyridine ring was not destroyed over the potential range from −0.8 to 1 V (vs. SCE), and the electrocatalytic generation of pyridoxal was anodically started at 0.57 V.