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MicroITIES Detection of Adenosine Phosphates
Author(s) -
Qian Quansheng,
Wilson George S.,
BowmanJames Kristin
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200302959
Subject(s) - nucleotide , chemistry , moiety , gibbs free energy , aqueous solution , phosphate , ligand (biochemistry) , ion , adenosine , amine gas treating , differential pulse voltammetry , adenosine triphosphate , cyclic voltammetry , stereochemistry , inorganic chemistry , electrochemistry , organic chemistry , electrode , biochemistry , receptor , thermodynamics , physics , gene
The complexation between a triamide ligand derived from tris‐2‐(aminoethyl)amine: N ‐{2‐[bis‐(2‐(4‐ tert ‐butylbenzoyl)‐aminoethyl)‐amino]‐ethyl}4‐ tert ‐butylbenzamide, L , and the three adenosine‐containing nucleotides, ATP, ADP, and AMP, was investigated by facilitated ion transfer processes through a microhole array film. Differential pulse voltammetry (DPV) was used to measure the transfer currents for the respective nucleotides. The three nucleotides were found to have different transfer potentials with transfer currents proportional to their aqueous concentrations. Based on the differences of the transfer potentials, it is concluded that the host ligand, L, interacts with the phosphate moiety and the Gibbs transfer energy is dominated by the charge generated by the phosphate groups. The linear relationship between the current response and nucleotide concentration forms the basis of an anion sensor with a dynamic range from 0.1 m M to 5 m M .