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Probing DNA Hybridization by Impedance Measurement Based on CdS‐Oligonucleotide Nanoconjugates
Author(s) -
Xu Ying,
Cai Hong,
He PinGang,
Fang YuZhi
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200302923
Subject(s) - oligonucleotide , dielectric spectroscopy , monolayer , dna , covalent bond , electrode , dna–dna hybridization , materials science , biosensor , electron transfer , hybridization probe , nucleic acid thermodynamics , chemistry , oligomer restriction , analytical chemistry (journal) , electrochemistry , nanotechnology , photochemistry , biochemistry , chromatography , base sequence , organic chemistry
A novel, sensitive DNA hybridization detection protocol, based on DNA‐quantum dots nanoconjugates coupled with electrochemical impedance spectroscopy (EIS) detection, is described. The amino‐linked ss‐DNA probe was covalently immobilized onto a self‐assembled mercaptoacetic acid monolayer modified gold electrode; after hybridization with the target ssDNA‐CdS nanoconjugates, EIS was used to detect the change of interfacial electron‐transfer resistance ( R et ) of the redox marker, [Fe(CN) 6 ] 4−/3− , from solution to transducer surface. The results showed that when target ssDNA‐CdS nanoconjugates hybridized with probe oligonucleotide, a double helix film formed on the electrode, a remarkably increased R et value was observed. Only complementary DNA sequence had an obvious signal compared with three‐base mismatched or non‐completely matched sequences under the optimized experimental conditions. Due to having more negative charges, space resistance and the semiconductor property, CdS nanoparticle labels on target DNA could improve the sensitivity to two orders of magnitude when compared with non‐CdS tagged DNA sequences.