Premium
Determination of Cytochrome C with Cellulose – DNA Modified Carbon Paste Electrodes
Author(s) -
Lee TaeYoung,
Kim HwaJeong,
Moon JeonOk,
Shim YoonBo
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200302885
Subject(s) - detection limit , carbon paste electrode , cellulose , cytochrome c , differential pulse voltammetry , analytical chemistry (journal) , electrode , graphite , calibration curve , chemistry , adsorption , materials science , nuclear chemistry , cyclic voltammetry , chromatography , electrochemistry , organic chemistry , biochemistry , mitochondrion
A sensor for cytochrome c (cyt c) was developed using a carbon paste electrode (CPE) modified with cellulose‐DNA. Cyt c was adsorbed on the cellulose‐DNA modified CPE through the electrostatic interaction between them. Owing to this process, a pair of well‐defined peaks appeared at +48 mV/85 mV ( E pc / E pa vs. Ag/AgCl). This property of the cellulose‐DNA modified CPE was utilized for the analysis of cyt c in a biological sample. The optimum experimental conditions for analysis were investigated and a calibration plot was obtained between 1.0×10 −4 M and 1.0×10 −6 M (±5% at n =5) at the optimized condition. The detection limit for cyt c at the optimized experimental condition was 5.0×10 −7 M (S/N=3) for 30 min of deposition time with differential pulse voltammetry (DPV). The real sample analysis was carried out with the standard addition method to evaluate the developed method. The content of cyt c in total proteins of 80.0 mg/mL in rat mitocondria fractions was determined to be 0.12 (±0.02) mg/mL.