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Detecting DNA Damage with a Silver Solid Amalgam Electrode
Author(s) -
Kuchariková Katerina,
Novotny Ladislav,
Yosypchuk Bogdan,
Fojta Miroslav
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200302874
Subject(s) - electrode , dna , mercury (programming language) , amalgam (chemistry) , materials science , cyclic voltammetry , biosensor , dna supercoil , chemistry , nanotechnology , electrochemistry , biochemistry , computer science , dna replication , programming language
Mercury electrodes modified with supercoiled (sc) DNA have been used as highly sensitive tools for the detection of DNA strand breaks or as sensors for DNA cleaving substances. In this paper we show that silver solid amalgam electrode (AgSAE), in connection with alternating current voltammetry, provides similar information about DNA damage as the hanging mercury drop electrode. The AgSAE can be used for the detection of enzymatic or chemical DNA cleavage in solution or at the electrode surface. AgSAE modified with scDNA can be utilized as a sensor for DNA nicking substances.