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Investigation by QCM of the Specific and Nonspecific Avidin Interaction onto a Biotinylated Polypyrrole Film
Author(s) -
DupontFilliard A.,
Billon M.,
Bidan G.,
Guillerez S.
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200302867
Subject(s) - polypyrrole , quartz crystal microbalance , avidin , biotinylation , adsorption , materials science , polymer , conductive polymer , chemistry , chemical engineering , organic chemistry , composite material , biochemistry , polymerization , engineering
We have studied by gravimetric measurements using a quartz crystal microbalance (QCM), the adsorption of avidin onto a polypyrrole film. From QCM results, it appears that both the thickness and the redox state of polypyrrole strongly influence the avidin adsorption. This nonspecific interaction is larger for a thick film than for a thin one and, also for when the polymer is in its reduced state. We have also investigated by QCM the specific and nonspecific interaction of avidin onto biotinylated polypyrrole films having either a hydrophilic or hydrophobic character. It is shown that the amount of avidin immobilized by specific recognition is almost the same whatever the nature of the polymer matrix.