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Lead Sulfide Nanoparticle as Oligonucleotides Labels for Electrochemical Stripping Detection of DNA Hybridization
Author(s) -
Zhu Ningning,
Zhang Aiping,
Wang Qingjiang,
He Pingang,
Fang Yuzhi
Publication year - 2004
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200302835
Subject(s) - oligonucleotide , detection limit , anodic stripping voltammetry , stripping (fiber) , nanoparticle , lead sulfide , chemistry , electrochemistry , oligomer restriction , sulfide , dna–dna hybridization , hybridization probe , cyclic voltammetry , voltammetry , combinatorial chemistry , dna , electrode , inorganic chemistry , materials science , chromatography , nanotechnology , organic chemistry , biochemistry , composite material , quantum dot
We report a method for the detection of DNA hybridization in connection to lead sulfide (PbS) nanoparticle tags and electrochemical stripping measurement of the lead. A kind of lead sulfide nanoparticle with free carboxyl groups on its surface was synthesized in aqueous solution. The nanoparticle was used as a marker to label a sequence‐known oligonucleotide, which was then employed as a DNA probe for identifying a target ssDNA immobilized on a PPy modified electrode based on a specific hybridization reaction. The hybridization events were monitored by the oxidation dissolution of the lead sulfide anchored on the hybrids and the indirect determination of the lead ions by anodic stripping voltammetry (ASV). The detection limit is 0.3 pmol L −1 of target oligonucleotides. The PbS nanoparticle combining its easy conjugation to the DNA molecule with the highly sensitive stripping voltammetry detection of lead shows its promising application in the electrochemical DNA hybridization analysis assay.