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Rapid Amperometric Immunoassay for Escherichia coli Based on Graphite Coated Nylon Membranes
Author(s) -
Shah Jasmin,
Chemburu Sireesha,
Wilkins Ebtisam,
AbdelHamid Ihab
Publication year - 2003
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200302791
Subject(s) - amperometry , detection limit , immunoassay , graphite , membrane , chromatography , materials science , electrode , chemistry , electrochemistry , composite material , biochemistry , antibody , immunology , biology
An amperometric immunosensor for rapid detection of bacteria has been developed. A graphite‐coated nylon membrane serves as a support for antibody immobilization and as a working electrode. The different parameters affecting sensor preparation such as, selection of the conductive material, pore size of the nylon membrane, number of coatings and the ratios of the graphite to the binder were optimized. A “sandwich” immunoassay scheme was used and amperometric detection of captured bacteria was conducted by the electro‐reduction of iodine formed as the product of a peroxidase catalyzed reaction. This approach has been used for detection of total Escherichia coli with a low detection limit of 40 cells/mL. The developed system can be easily adapted for rapid analysis of different pathogenic microorganisms.