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Composite Multienzyme Amperometric Biosensors for an Improved Detection of Phenolic Compounds
Author(s) -
Serra B.,
Reviejo A. J.,
Pingarrón J. M.
Publication year - 2003
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200302765
Subject(s) - biosensor , glucose oxidase , amperometry , chemistry , tyrosinase , peroxidase , immobilized enzyme , matrix (chemical analysis) , chromatography , graphite , nuclear chemistry , organic chemistry , electrode , electrochemistry , enzyme , biochemistry
A biosensor design, in which glucose oxidase and peroxidase are coimmobilized by simple physical inclusion into the bulk of graphite‐Teflon pellets, is reported for the detection of phenolic compounds. This design allows the “in situ” generation of the H 2 O 2 needed for the enzyme reaction with the phenolic compounds, which avoids several problems detected in the performance of single peroxidase biosensors as a consequence of the presence of a high H 2 O 2 concentration. So, a much lower surface fouling was found at the GOD‐HRP biosensor in comparison with a graphite‐Teflon‐HRP electrode, suggesting that the controlled generation of H 2 O 2 makes more difficult the formation of polymers from the enzyme reaction products. The construction of trienzyme biosensors, in which GOD, HRP and tyrosinase were coimmobilized into the graphite‐Teflon matrix is also reported, and their performance was compared with that of GOD‐HRP bienzyme electrodes. The practical applicability of the composite multienzyme amperometric biosensors was evaluated by the estimation of the phenolic compounds content in waste waters from a refinery, and the results were compared with those obtained by using a colorimetric official method based on the reaction with 4‐aminoantipyrine.