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PCR‐Genosensor Rapid Test for Detecting Salmonella
Author(s) -
Pividori M. Isabel,
Merkoçi Arben,
Barbé Jordi,
Alegret Salvador
Publication year - 2003
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200302764
Subject(s) - amplicon , chromatography , salmonella , chemistry , detection limit , dna , microbiology and biotechnology , polymerase chain reaction , biochemistry , biology , gene , genetics , bacteria
A PCR‐genosensor based assay for detecting the IS200 DNA sequence specific to Salmonella spp is reported. This rapid test is based on PCR, where amplicon detection is achieved electrochemically using GEC (graphite‐epoxy composite) genosensor. The amplicon is immobilized onto GEC electrodes by simple dry‐adsorption and the detection is carried out using an enzymatic labeling system. Results demonstrate that this new electrochemical genosensor fulfils the requirements desired for these devices: easy preparation – as dry‐adsorption of DNA is very simple –, robustness, sensitivity, low cost, simple use and fast response. Additionally, the assay can be produced as a kit format, increasing its commercial potentiality. Also, the electrode material can be implemented for screen‐printing procedures for the mass production of genosensors.