A New Polyphenol Oxidase Biosensor Mediated by Azure B in Laponite Clay Matrix | Zendy

Shan Dan | Zendy; Mousty Christine | Zendy; Cosnier Serge | Zendy; Mu Shaolin | Zendy

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A New Polyphenol Oxidase Biosensor Mediated by Azure B in Laponite Clay Matrix

Author(s) -

Shan Dan,

Mousty Christine,

Cosnier Serge,

Mu Shaolin

Publication year - 2003

Publication title -

electroanalysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.574

H-Index - 128

eISSN - 1521-4109

pISSN - 1040-0397

DOI - 10.1002/elan.200302740

Subject(s) - glutaraldehyde , biosensor , catechol , polyphenol oxidase , chemistry , phenol , matrix (chemical analysis) , amperometry , cationic polymerization , tyrosinase , glucose oxidase , nuclear chemistry , chromatography , chemical engineering , organic chemistry , electrode , enzyme , electrochemistry , biochemistry , engineering , peroxidase

Amperometric biosensor based on the entrapment of polyphenol oxidase within a laponite clay coating and cross‐linked by glutaraldehyde is described for catechol detection. Laponite provides a hydrophilic enzyme surrounding increasing the long term stability of the biosensor compared to the corresponding biosensors obtained by chemical cross‐linking of PPO with glutaraldehyde. Azure B, a cationic dye exchanged within the clay matrix, is used as an electron shuttle allowing the mediated detection of phenol derivatives at −0.05 V. The detection limits obtained with the optimized biosensor configuration for catechol, p ‐cresol and phenol are 1, 1 and 17 nM, respectively.

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