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A New Polyphenol Oxidase Biosensor Mediated by Azure B in Laponite Clay Matrix
Author(s) -
Shan Dan,
Mousty Christine,
Cosnier Serge,
Mu Shaolin
Publication year - 2003
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.200302740
Subject(s) - glutaraldehyde , biosensor , catechol , polyphenol oxidase , chemistry , phenol , matrix (chemical analysis) , amperometry , cationic polymerization , tyrosinase , glucose oxidase , nuclear chemistry , chromatography , chemical engineering , organic chemistry , electrode , enzyme , electrochemistry , biochemistry , engineering , peroxidase
Amperometric biosensor based on the entrapment of polyphenol oxidase within a laponite clay coating and cross‐linked by glutaraldehyde is described for catechol detection. Laponite provides a hydrophilic enzyme surrounding increasing the long term stability of the biosensor compared to the corresponding biosensors obtained by chemical cross‐linking of PPO with glutaraldehyde. Azure B, a cationic dye exchanged within the clay matrix, is used as an electron shuttle allowing the mediated detection of phenol derivatives at −0.05 V. The detection limits obtained with the optimized biosensor configuration for catechol, p ‐cresol and phenol are 1, 1 and 17 nM, respectively.