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Mesoporous TiO 2 films: New catalytic electrode fabricating amperometric biosensors based on oxidases
Author(s) -
Cosnier Serge,
Gondran Chantal,
Senillou Anne,
Grätzel Michael,
Vlachopoulos Nicholas
Publication year - 1997
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140091803
Subject(s) - glutaraldehyde , amperometry , glucose oxidase , biosensor , mesoporous material , hydrogen peroxide , immobilized enzyme , detection limit , electrode , catalysis , aqueous solution , materials science , chemical engineering , chemistry , nanotechnology , nuclear chemistry , chromatography , organic chemistry , electrochemistry , enzyme , engineering
A novel catalytic material (mesoporous TiO 2 films) for the cathodic detection of hydrogen peroxide in air‐saturated aqueous solution is described. The immobilization of glucose oxidase (GOD) as enzyme model onto the TiO 2 electrode provides an efficient biosensor for the amperometric detection of glucose at −0.15 V (vs. SCE). GOD molecules were immobilized in an inorganic laponite gel and cross‐linked by glutaraldehyde. The highest sensitivity and detection limit were 3.33 mAM −1 cm −2 and 15 μM, respectively. In addition different procedures of enzyme immobilization including cross‐linking with glutaraldehyde and physical entrapment in a functionalized polypyrrole film have been compared.
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