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Immobilization of methylene blue using α‐zirconium phosphate and its application within a reagentless amperometric hydrogen peroxide biosensor
Author(s) -
Ruan Chuanmin,
Yang Feng,
Xu Jinsuo,
Lei Chengheng,
Deng Jiaqi
Publication year - 1997
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140091511
Subject(s) - methylene blue , hydrogen peroxide , amperometry , biosensor , zirconium phosphate , chemistry , inorganic chemistry , phosphate , zirconium , zirconium oxide , combinatorial chemistry , nuclear chemistry , catalysis , electrochemistry , organic chemistry , electrode , biochemistry , photocatalysis , oxide
A novel H 2 O 2 sensor was constructed by employing methylene blue (MB) incorporated in α‐zirconium phosphate as an electron transfer mediator. Methylene blue was intercalated into a α‐zirconium phosphate modified horseradish peroxidase (HRP) immobilization matrix cross‐linked on a glassy carbon electrode surface via bovine serum albumin (BSA)‐glutaraldehyde. Such a coimmobilization matrix of the mediator and enzyme was formed from α‐zirconium phosphate‐MB inclusion colloid in which horseradish peroxidase was dissolved. Intercalation of methylene blue in layered α‐zirconium phosphate was investigated by X‐ray power diffraction (XRD), IR spectra and electrochemical measurements. Methylene blue immobilized in this way underwent a quasi‐reversible electrochemical redox reaction. Cyclic voltammetry and amperometric measurements demonstrated that methylene blue coimmobilized with HRP displayed good stability and efficiently shuttled electrons between HRP and the electrode. The sensor responded rapidly and linearly to H 2 O 2 over the range from 2.0 × 10 −6 to 5.0 × 10 −3 mol/L, with a detection limit of 3.0 × 10 −7 mol/L.