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A bienzyme sensor for the determination of hypoxanthine and inosine
Author(s) -
Hu Shengshui,
Liu ChungChiun
Publication year - 1997
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140091510
Subject(s) - hypoxanthine , inosine , purine nucleoside phosphorylase , biosensor , xanthine oxidase , chemistry , nafion , electrode , phosphate , clark electrode , chromatography , enzyme , electrochemistry , biochemistry , purine , electrolyte
A biosensor for the detection of hypoxanthine (Hx) and inosine (HxR) has been developed by the coimmobilization of nucleoside phosphorylase (NP) and xanthine oxidase (XO) on a chemically modified electrode, which uses a glassy carbon electrode coated with a Nafion ‐ paraquat film. This sensor responds to Hx and HxR in the presence of phosphate, and it responds only to Hx in the absence of phosphate (borate buffer as medium). The sensor measurement is based on the oxygen consumed due to the enzymatic reaction involving xanthine oxidase which is immobilized on the electrode surface. In this study, the experimental parameters are optimized and the linear relationships between the decreased currents and the concentrations of both Hx and HxR in the range of 1 × 10 −6 ‐2 × 10 −4 M are obtained. This biosensor exhibits a high degree of sensitivity and can be used for the assay for Hx and HxR in fish meat or other biological samples.