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Development of a sol‐gel enzyme inhibition‐based amperometric biosensor for cyanide
Author(s) -
Park TaeMyung,
Iwuoha Emmanuel I.,
Smyth Malcolm R.
Publication year - 1997
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140091416
Subject(s) - chemistry , amperometry , cyanide , horseradish peroxidase , hydrogen peroxide , thiourea , detection limit , sodium azide , biosensor , prussian blue , inorganic chemistry , potassium cyanide , nuclear chemistry , chromatography , electrochemistry , electrode , organic chemistry , enzyme , biochemistry
Abstract An amperometric sol‐gel‐based enzyme inhibition electrode for the detection of low levels of cyanice was constructed by immobilizing horseradish peroxidase (HRP) and an osmium redox polymer ([Os(bpy) 2 (PVP) 10 Cl]Cl; abbreviated Os‐polymer) as mediator. Upon addition of hydrogen peroxide to the solution, a bioelectrocatalytic reduction wave was observed, which was diffusion controlled. This reduction current is subsequently inhibited by cyanide. The steady‐state hydrogen peroxide catalytic reduction current reached a plateau at 150 mV (vs. Ag/AgCl) and cyanide could be determined amperometrically with good sensitivity at 0mV (vs. Ag/AgCl). The concentration range of linear response, apparent inhibition constant ( K ), and detection limit were 0.004–0.04 mM, 0.0637 mM, and 0.5 μM, respectively. Sodium azide, potassium thiocyanide and thiourea were all found not to interfere at this potential. The biosensor was found to be stable for over 1 week on storage at room temperature.