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Acetylcholine minisensor based on metal‐supported lipid bilayers for determination of environmental pollutants
Author(s) -
Rehák M.,
Šnejdárková M.,
Hianik T.
Publication year - 1997
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140091408
Subject(s) - choline oxidase , chemistry , acetylcholinesterase , choline , acetylcholine , amperometry , fluoride , membrane , chromatography , inorganic chemistry , biochemistry , electrochemistry , enzyme , electrode , medicine , endocrinology
Immobilization of avidin‐modified acetylcholinesterase and choline oxidase (A‐COxACE) on a metal supported biotinylated membrane allowed us to developed a minisensor based on changes in anticholinesterase activity by several environmental pollutants. The concentrations of acetylcholine and choline have been determined amperometrically on the basis of anodic oxidation of hydrogen peroxide, a product of choline oxidase. The immobilization of the complex A COxACE was controlled by the electrostriction method which allowus to determine changes of membrane capacitance C and elasticity modulus E ⟂ in a direction perpendicular to the s‐BLM surface. The binding of the A‐COxACE complex to s‐BLM resulted in a decrease of C and in an increase of E ⟂ suggesting increase in the membrane ordering. Using the simple amperometric method we were able to use this minisensor for detection of acetylcholine and choline. Detection limits were 0.79 mM and 1.25 mM for acetylcholine and choline, respectively. The biosensor was tested for acetylcholinesterase inhibition in the presence of organophosphorus Trichlorphon and eserine.