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Direct electron transfer reaction of glucose oxidase at bare silver electrodes and its application in analysis
Author(s) -
Wen Zhihui,
Ye Baoxian,
Zhou Xingyao
Publication year - 1997
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140090812
Subject(s) - electron transfer , glucose oxidase , electrochemistry , electrode , chemistry , redox , adsorption , reaction rate constant , analytical chemistry (journal) , inorganic chemistry , photochemistry , kinetics , organic chemistry , physics , quantum mechanics
The direct electron transfer reaction of glucose oxidase (GOx) at a bare silver electrode is verified. The electron transfer number n = 2, electron transfer coefficient α = 0.45 and rate constant of the electrochemical reaction K s = 0.1 s −1 are obtained. This communication presents a multimolecular adsorption model to explain the properties of the direct electron reaction between GOx and bare silver electrodes. The residual valence force may be an important factor to ensure a direct electron transfer reaction on the bare electrode. On the basis of the experimental fact that only biologically active GOx exhibits electrochemical activity in solution, a facile analytical method for analyzing the active GOx concentration is developed. The results determined correspond very well to that of a spectrometric method.