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Biodegradation of soluble redox polymers. Part 2: (0.017ferrocene)amylose at a rotating disk electrode
Author(s) -
Gnedenko Boris B.,
Galkin Alexander M.,
Ryabov Alexander D.
Publication year - 1997
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140090803
Subject(s) - chemistry , amylose , depolymerization , ferrocene , rotating disk electrode , redox , enzyme , cyclic voltammetry , limiting current , limiting , electrode , analytical chemistry (journal) , chromatography , nuclear chemistry , inorganic chemistry , organic chemistry , electrochemistry , starch , mechanical engineering , engineering
The behavior of (ferrocene)amylose (FA), in the presence of amylolytic depolymerases (α‐amylase from Aspergillus oryzae and human saliva), has been investigated by cyclic voltammetry at a rotating disk electrode (CVA/RDE). Growth of the limiting current with time in the presence of the enzymes is proportional to the amount of enzyme introduced. The quantitative data treatment to assay the endoamylolytic activity of enzymes at CVA/RDE involves plotting ( i dt / i d0 ) 4.5 against time; the slope of the linear plot being equal to (rate) M n0 C −1 , where i dt and i d0 are the limiting currents at time t and 0, respectively, ( rate ) is the enzymatic activity, M n0 is the number averaged molecular weight of FA at t = 0, and c is its concentration. The comparison of CVA/RDE with the 3,5‐dinitrosalicylic acid and the Somogyi–Nelson reducing saccharides procedures shows advantages of the former, especially in assaying small quantities of enzymes. Also the CVA/RDE approach is simpler and takes place under much milder conditions. The main disadvantage of CVA/RDE is the inhibiting effect of Triton X‐100 in the reaction between FA and the amylases which is not observed in the case of native, ferrocene‐free amylose. In general, CVA/RDE appears to be an attractive analytical method for monitoring diverse enzymatic depolymerization reactions.