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Development of a hypoxanthine biosensor based on immobilized xanthine oxidase chemically modified electrode
Author(s) -
Hu Shengshui,
Liu ChungChiun
Publication year - 1997
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140090504
Subject(s) - hypoxanthine , biosensor , xanthine oxidase , nafion , amperometry , chemistry , electrode , clark electrode , immobilized enzyme , xanthine , selectivity , chromatography , electrochemistry , catalysis , organic chemistry , biochemistry , enzyme , electrolyte
An amperometric biosensor for hypoxanthine determination has been developed. The sensor uses a Nafion‐paraquat chemically modified glassy‐carbon electrode. It detects the oxygen consumed by the enzymatic reaction catalyzed by xanthine oxidase which is immobilized on the electrode surface. The sensor responds linearly to hypoxanthine over the concentration range of 1 × 10 −6 M −2 × 10 −4 M. The biosensor can be reused for more than 100 times without significant deterioration in performance. After 32 days storage at 3–5°C, the sensor response remains at 68% of the initial level. The high sensitivity, selectivity and stability of this biosensor demonstrates its practical applicability for a simple, rapid and economical determination of hypoxanthine in fish samples.