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D ‐Fructose sensing electrode based on electron transfer of D ‐fructose dehydrogenase at colloidal gold‐enzyme modified electrode
Author(s) -
Yabuki Soichi,
Mizutani Fumio
Publication year - 1997
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140090107
Subject(s) - electrode , fructose , electron transfer , dehydrogenase , enzyme , chemistry , biochemistry , photochemistry
A complex of colloidal gold (Au) particles and D‐fructose dehydrogenase was prepared. A glassy carbon (GC) electrode was modified with the complex. The anodic current response to D‐fructose was measured by using the GC electrode modified with the complex at +0.5 V (vs. Ag/AgCl). Response time was within 15 s. No current response was observed in the absence of enzyme, so that the increased oxidation current was confirmed to be caused by the electron transfer from enzyme to electrode. Linear response range of the electrode was up to 0.5 mM fructose concentration. Fructose concentrations of soft drinks which were determined using the electrode agreed well with the results of the F‐kit.