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Potentiometric determination of total and ionized calcium in osteoblast‐like cell culture medium
Author(s) -
Morais Simone,
Carvalho Graça S.,
Sousa João P.
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140081220
Subject(s) - potentiometric titration , chemistry , calcium , ionic strength , sodium , electroanalytical method , bicarbonate , ionization , sodium bicarbonate , inorganic chemistry , electrode , detection limit , chromatography , analytical chemistry (journal) , ion , organic chemistry , aqueous solution
Abstract The effect of the ionic strength and composition of solutions in the direct potentiometric determination of total and ionized calcium content using a calcium selective electrode was investigated. In the presence of a constant complexation buffer (CCB), the addition of sodium chloride and sodium bicarbonate causes a decrease in the limit of detection of the potentiometric response. When the procedure was tested in osteoblast‐like cell culture medium no electrode interferences or method interferences were observed by the presence of proteins, i.e., the total and ionized calcium in this medium can be measured by potentiometric means.