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The effect of organic solvent properties on a catalase enzyme sensor for monitoring hydrogen peroxide in nonaqueous solutions
Author(s) -
Campanella Luigi,
Martini Umberto,
Sammartino Maria P.,
Tomassetti Mauro
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140081215
Subject(s) - hydrogen peroxide , anhydrous , chemistry , solvent , chloroform , catalase , biosensor , organic solvent , inorganic chemistry , dielectric , organic chemistry , enzyme , chemical engineering , materials science , biochemistry , optoelectronics , engineering
A catalase based biosensor for determining hydrogen peroxide in organic solvents, operating directly in nonaqueous solutions, was prepared, characterized (using water saturated chloroform as solvent) and applied in comparative tests using four different anhydrous organic solvents in order to determine their effect on biosensor sensitivity and thus on enzymatic activity in different organic solvents. Results are discussed in terms of log P and dielectric constant (DEC) values of the solvents considered.