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Immobilization of pyrroloquinoline quinone on gold by carbodiimide coupling to adsorbed polyamines: Stability comparison to attachment via a chemisorbed thiol monolayer
Author(s) -
Moore Andrew N. J.,
Katz Eugenii,
Willner Itamar
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140081203
Subject(s) - pyrroloquinoline quinone , carbodiimide , thiol , monolayer , chemistry , adsorption , coupling (piping) , photochemistry , polymer chemistry , organic chemistry , materials science , biochemistry , enzyme , cofactor , metallurgy
Pyrroloquinoline quinone (PQQ) was immobilized on gold electrodes by prior adsorption of the polyamines polyethylenimine, and triethylene tetraamine, followed by carbodiimide coupling of PQQ to the surface‐adsorbed amine groups. The stability and electrochemical behavior of the electrodes, in which the amino‐containing linker molecule is adsorbed on the electrode surface, were compared with those of electrodes prepared by carbodiimide coupling of PQQ to a cystamine monolayer, in which the sulfur‐containing linker molecule is associated with the gold surface. The electrodes prepared using adsorbed polyethylenimine showed comparable stability to those based on a cystamine monolayer, but reveal enhanced stability to application of negative potentials (< −1.1 V). The method provides an alternative to the conventional functionalization of Au electrodes by thiols.