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Amperometric alcohol sensors based on protein multilayers composed of avidin and biotin‐labeled alcohol oxidase
Author(s) -
Du Xiaoyan,
Anzai Junichi,
Osa Tetsuo,
Motohashi Ryoichi
Publication year - 1996
Publication title -
electroanalysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.574
H-Index - 128
eISSN - 1521-4109
pISSN - 1040-0397
DOI - 10.1002/elan.1140080820
Subject(s) - alcohol oxidase , amperometry , chemistry , alcohol , ethanol , methanol , avidin , butanol , selectivity , biosensor , biotin , chromatography , platinum , inorganic chemistry , analytical chemistry (journal) , electrode , biochemistry , organic chemistry , electrochemistry , catalysis , gene , recombinant dna , pichia pastoris
Protein multilayers composed of avidin and biotin‐labeled alcohol oxidase (AOx) were prepared on the surface of a platinum (Pt) electrode by repeated deposition of the proteins to develop amperometric alcohol sensors. The AOx multilayer‐modified sensors showed a rapid response to ethanol over the concentration range of 0.01–10mg/100mL. The output current of the sensor depended linearly upon the thickness of the AOx layers (or the number of the AOx layers), suggesting that a constant amount of AOx can be immobilized by each deposition. The selectivity of the sensor to aliphatic alcohols was in the order of methanol > ethanol > n ‐propanol > n ‐butanol, being in accord with the selectivity of AOx in a homogeneous system itself. The sensor retained its original response to ethanol for about one month without deterioration.